Poly(ADP-Ribose) Polymerase : Methods and Protocols - Tulin, Alexei V.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry -- Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies -- Detecting and Quantifying pADPr In Vivo -- Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools -- Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry -- Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe -- ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method -- Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs -- Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a 'Clickable' NAD+ Analog -- Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography - Tandem Mass Spectrometry -- Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography -Tandem Mass Spectrometry -- Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair -- Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function -- Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay -- Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1) - mRNA Targets by PAR-CLiP -- Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin -- PARP-1 Interaction with and Activation by Histones and Nucleosomes -- Strategies Employed for the Development of PARP Inhibitors -- High-Throughput Colorimetric Assay for Identifying PARP-1 Inhibitors Using a Large Small-Molecule Collection -- Testing PARP Inhibitors Using a Subcutaneous Murine Xenograft Model -- In Vitro Long Term Proliferation Assays to Study Antiproliferative Effects of PARP Inhibitors on Cancer Cells -- Use of Inosine Monophosphate Dehydrogenase Activity Assay to Determine the Specificity of PARP-1 Inhibitors -- The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy, Use as a Single Agent in Susceptible Patients, and Techniques Used to Identify Susceptible Patients -- Purification of Recombinant Human PARP-3 -- Purification of Recombinant Human PARG and Activity Assays -- Studying Catabolism of Protein ADP-Ribosylation -- Purification of DNA Damage-Dependent PARPs from E. coli for Structural and Biochemical Analysis -- Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach -- Computational and Experimental Studies of ADP-Ribosylation.
Keywords: Proteins .; Protein Science