Phagocytosis and Phagosomes : Methods and Protocols - Botelho, Roberto; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Phagocytosis: Hungry, Hungry Cells -- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry -- Quantitative Efferocytosis Assays -- Quantifying Phagocytosis by Immunofluorescence and Microscopy -- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles -- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis -- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers -- Quantitative Immunofluorescence to Study Phagosome Maturation -- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis -- Analysis of LC3-associated Phagocytosis and Antigen Presentation -- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells -- Measuring Phagosomal pH by Fluorescence Microscopy -- Image-Based Analysis of Phagocytosis - Measuring Engulfment and Internalization -- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes -- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging -- <isolation and="" western="" blotting="" of="" latex-bead="" phagosomes="" to="" track="" phagosome="" maturation.-="" assessing="" the="" proteome="" by="" quantitative="" mass="" spectrometry.-="" dissecting="" phagocytic="" removal="" apoptotic="" cells="" in="" Caenorhabditis Elegans -- Measurement of Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells -- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units -- Filamentous Bacteria as Targets to Study Phagocytosis -- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays -- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome -- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>.
Keywords: Microbiology; Microbiology