Plant Kinases : Methods and Protocols - Dissmeyer, Nico.; Schnittger, Arp.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Guide to the Book Plant Kinases -- The Age of Protein Kinases -- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts -- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System -- Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants -- Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks -- Bacterial Assay to Study Plant Sensor Histidine Kinases.-  Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase -- Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen -- Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules -- Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization -- Quantifying Degradation Rates of Trans-Membrane Receptor Kinases -- Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta -- Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo -- Chemical Genetic Analysis of Protein Kinase Function in Plants -- Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes -- Phosphoproteomics Using iTRAQ.
Keywords: Plant science; Botany; Proteomics; Plant Sciences; Proteomics