RNA : Methods and Protocols - Nielsen, Henrik; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
The Transcriptional Landscape -- Working with RNA -- Synthesis of RNA by In vitro Transcription -- Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capture -- Genome Browsers -- Web-Based Tools for Studying RNA Structure and Function -- Northern Blotting Analysis -- Rapid Amplification of cDNA Ends (RACE) -- Fractionation of mRNA Based on the Length of the Poly(A) Tail -- Analysis of Mutations that Influence Pre-mRNA Splicing -- S1 Nuclease Analysis of Alternatively Spliced mRNA -- Promises and Challenges in Developing RNAi as a Research Tool and Therapy -- Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA) -- Validation of RNAi by Real Time PCR -- Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method -- The Post-Transcriptional Operon -- RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling -- Isolation of RNP Granules -- Electrophoretic Mobility Shift Assay for Characterizing RNA-Protein Interaction -- Polysome Analysis and RNA Purification from Sucrose Gradients -- Prediction of Targets for microRNAs -- Outsourcing of Experimental Work -- ESM1: In-Line Probing Analysis of Riboswitches -- ESM2: Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE).
Keywords: Human genetics; Gene expression; Human Genetics; Gene Expression