Plant Proteostasis : Methods and Protocols - Lois, L. Maria; Matthiesen, Rune.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Approaches to Determine Protein Ubiquitination Residue Types -- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis Thaliana Seedlings -- Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors -- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes -- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants -- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro -- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases -- SUMO Chain Formation by Plants Enzymes -- Kinetic Analysis of Plant SUMO Conjugation Machinery -- Expression, Purification, and Enzymatic Analysis Of Plant SUMO Proteases -- Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging -- Biochemical Analysis of Autophagy in Algae and Plants by Monitoring the Electrophoretic Mobility of ATG8 -- Detection of Autophagy in Plants by Fluorescence Microscopy -- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System -- Detection and Quantification of Protein Aggregates in Plants -- Determination of Protein Carbonylation and Proteasome Activity in Seeds -- Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate Affinity Chromatography (PIP - chromatography) for the Isolation of Proteins Involved in Protein Quality Control and Proteostasis Mechanisms in Plants -- In vivo Radiolabeling of Arabidopsis Leaves and Separation of Thylakoid Membrane Complexes by Blue Native PAGE -- Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling -- Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes using CoGe -- Studying Evolutionary Dynamics of Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest -- Bioinformatics Tools for Exploring the SUMO Gene Network. .
Keywords: Plant science; Botany; Plant Sciences