Protein Electrophoresis : Methods and Protocols - Kurien, Biji T.; Scofield, R. Hal.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
How It All Began -- A Personal History of Gel-Electrophoresis -- Introduction to Protein Electrophoresis -- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis -- Protein Solubilization: Attend to the Choice of Lysis Buffer -- Native Polyacrylamide Gels -- Cationic Electrophoresis -- SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins -- Tricine-SDS-PAGE -- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis -- Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins -- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis -- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples -- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies -- Microchip Electrophoresis, with respect to "Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" -- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols -- Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE -- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass -- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting -- Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing -- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids -- Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution -- Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE -- One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis -- Two-Dimensional Difference Gel Electrophoresis -- Protein Identification on Archived 2-D Gels -- Diagonal Electrophoresis for the Detection of Protein Disulfides -- Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes -- Immunoelectrophoresis: A Method with Many Faces -- Cell Surface Protein Biotinylation for SDS PAGE Analysis -- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization -- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome -- Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test -- Extraction of Proteins from Gels - A Brief Review -- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets -- Isolation of  Proteins from Polyacrylamide Gels -- Gel Drying Methods -- Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags -- A Brief Review of Other Notable Electrophoretic Methods -- Protein Stains and Applications -- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels -- Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat -- Silver Staining Techniques of Polyacrylamide Gels -- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis -- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry -- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE -- Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins -- Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping -- Fluorescent Staining of Gels -- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis -- Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain -- Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain -- In-Gel Staining of Proteins in Native Poly Acryl Amide Gel  Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin -- Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black -- Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels -- Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner -- A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels -- Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein™, ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains -- Common Artifacts and Mistakes Made in Electrophoresis.
Keywords: Proteins .; Proteomics; Electrophoresis; Protein Science; Proteomics; Electrophoresis