Peroxisomes : Methods and Protocols - Schrader, Michael; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation -- Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes -- Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays -- Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae -- Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid -- Analysis of Plasmalogen Synthesis in Cultured Cells -- Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies -- siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells -- Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals -- Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidine -- Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins -- Detection and Immunolabelling of Peroxisomal Proteins -- Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis -- Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes -- KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress -- Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed -- In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy -- Blue Native Page: Applications to Study Peroxisome Biogenesis -- In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes -- Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System -- The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells -- Detection of Ubiquitinated Peroxisomal Proteins in Yeast -- Assessing Pexophagy in Mammalian Cells -- Experimental Systems to Study Yeast Pexophagy -- Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells -- Study of Peroxisomal Protein Phosphorylation by Functional Proteomics -- Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings -- Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy -- Generation of Peroxisome-Deficient Somatic Animal Cell Mutants -- Clinical and Laboratory Diagnosis of Peroxisomal Disorders.
Keywords: Cell biology; Cell Biology