Polyadenylation in Plants : Methods and Protocols - Hunt, Arthur G.; Li, Qingshun Quinn; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Computational Analysis Of Plant Polyadenylation Signals -- Prediction of Plant mRNA Polyadenylation Sites -- Extraction of poly(A) Sites from Large-scale RNA-seq Data -- Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites -- Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm -- RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation -- Characterization Of Plant Polyadenylation Complexes By Using Tandem Affinity Purification -- In vitro Analysis Of Cleavage And Polyadenylation In Arabidopsis -- Production, Purification, and Assay of Recombinant Proteins for in vitro Biochemical Analyses of the Plant Polyadenylation Complex -- Detection of Disulfide Linkage By Chemical Derivatization And Mass Spectrometry -- Transient Expression Using Agroinfiltration To Study Polyadenylation In Plants -- A 3' RACE Protocol To Confirm Polyadenylation Sites -- Phage Display Library Screening for Identification of Interacting Protein Partners -- Genome-wide Determination Of Poly(A) Site Choice In Plants -- DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing -- Poly(A) Tag Library Construction from 10 ng Total RNA -- Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq.
Keywords: Plant science; Botany; Plant genetics; Medical genetics; Plant Sciences; Plant Genetics and Genomics; Gene Function