Neuropeptides : Methods and Protocols - Merighi, Adalberto; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
What Are Neuropeptides? -- Neuropeptide Localization in Non Mammalian Vertebrates -- Combined Light and Electron Microscopic Visualization of Neuropeptides and Their Receptors in Central Neurons -- Neuropeptide RNA Localization in Tissue Sections -- Intron-Specific Neuropeptide Probes -- Direct In Situ RT-PCR -- Laser Capture Microdissection and Quantitative-PCR Analysis -- Visualization of Peptide Secretory Vesicles in Living Nerve Cells -- Fluorescence Imaging with Single-Molecule Sensitivity and Fluorescence Correlation Spectroscopy (FCS) of Cell-Penetrating Neuropeptides -- Analysis of Neuro-Immune Interactions by an In Vitro Co-Culture Approach -- Electrophysiology -- Localization of Neuropeptides by Radioimmunoassay -- Reversed-Phase HPLC and Hyphenated Analytical Strategies for Peptidomics -- Neuropeptidomics: Mass Spectrometry-Based Qualitative and Quantitative Analysis -- Suppression Subtractive Hybridization -- Neuropeptide Microdialysis in Free-Moving Animals -- Antibody Microprobes for Detecting Neuropeptide Release -- Neuropeptidases -- Neuropeptide Autoantibodies Assay -- Intranasal Delivery of Neuropeptides -- Prodrug Design for Brain Delivery of Small- and Medium-Sized Neuropeptides -- Measurement of Phosphorothioate Oligodeoxynucleotide Antisense Transport Across the Blood-Brain Barrier -- Liposome-Encapsulated Neuropeptides for Site-Specific Microinjection -- Recombinant Adeno-Associated Viral Vectors -- De-Orphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay.
Keywords: Neurosciences; Neurochemistry; Neurosciences; Neurochemistry