Neurodegeneration : Methods and Protocols - Manfredi, Giovanni; Kawamata, Hibiki; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Introduction to Neurodegenerative Diseases and Related Techniques -- In vivo and In vitro Determination of Cell Death Markers in Neurons -- Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods -- Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex -- Isolation and Culture of Postnatal Spinal Motoneurons -- Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration -- Neural Differentiation of Induced Pluripotent Stem Cells -- Developing Yeast Models of Human Neurodegenerative Disorders -- Modeling Dopamine Neuron Degeneration in C. elegans -- Role of the Proteasome in Fly Models of Neurodegeneration -- Modeling Neurodegenerative Diseases in Zebrafish Embryos -- Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases -- Modeling Focal Cerebral Ischemia In vivo -- Pharmacological Models of Parkison's Disease in Rodents -- Behavioral Phenotyping of Mouse Models of Neurodegeneration -- Characterization of Amyloid Deposits in Neurodegenerative Diseases -- Detection of Protein Aggregates in Neurodegenerative Diseases -- Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures -- Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium -- Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue -- Monitoring Mitophagy in Neuronal Cell Cultures -- Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging -- Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices -- Imaging Presynaptic Exocytosis in Corticostriatal Slices -- Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1 -- Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice -- Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD -- Designing, Performing, and Interpreting a Microarray-based Gene Expression Study -- Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases -- Specific Gene Silencing using RNAi in Cell Culture -- Stem Cell Transplantation for Spinal Cord Neurodegeneration -- Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases.
Keywords: Neurosciences; Neurosciences