Protein Crystallography : Methods and Protocols - Wlodawer, Alexander; Dauter, Zbigniew; Jaskolski, Mariusz; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Expression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag -- Protein Crystallization -- Advanced Methods of Protein Crystallization -- The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability -- Crystallization of Membrane Proteins: and Overview -- Locating and Visualizing Crystals for X-ray Diffraction Experiments -- Collection of X-ray Diffraction Data from Macromolecular Crystals -- Identifying and Overcoming Crystal Pathologies: Disorder and Twinning -- Applications of X-ray Micro-beam for Data Collection -- Serial Synchrotron X-ray Crystallography (SSX) -- Time-Resolved Macromolecular Crystallography at Modern X-ray Sources -- Structure Determination Using X-ray Free-electron Laser Pulses -- Processing of XFEL Data -- Many Ways to Derivatize Macromolecules and their Crystals for Phasing -- Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX -- Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis -- Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography -- Acknowledging Errors: Advanced Molecular Replacement with Phaser -- Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems -- Radiation Damage in Macromolecular Crystallography -- Boxes of Model Building and Visualization -- Structure Refinement at Atomic Resolution -- Low Resolution Refinement of Atomic Models against Crystallographic Data -- Stereochemistry and Validation of Macromolecular Structures -- Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands -- Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank -- Databases, Repositories and Other Data Resources in Structural Biology.
Keywords: Proteins .; Protein Science