Nonribosomal Peptide and Polyketide Biosynthesis : Methods and Protocols - Evans, Bradley; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Structural Biology of Nonribosomal Peptide Synthetases -- The Assembly Line Enzymology of Polyketide Biosynthesis -- Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay -- Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains -- Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases -- Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis -- Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro -- The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis -- Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics -- Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi -- In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging -- Secondary Metabolic Pathway-Targeted Metabolomics -- Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra -- Bioinformatics Tools for the Discovery of New Nonribosomal Peptides -- The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways -- Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains -- Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
Keywords: Proteins .; Enzymology; Protein Science; Enzymology