Plant Vacuolar Trafficking : Methods and Protocols - Pereira, Cláudia.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds -- Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture -- Vacuolar Sorting Determinants - Isolation and Study -- Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies -- Plant Cell Vacuoles: Staining and Fluorescent Probes -- Vacuolar Targeting and Characterization of Recombinant Antibodies -- Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus -- Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells -- Live cell Imaging and Confocal Microscopy -- Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM) -- The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana -- Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana -- Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking -- Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay -- Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae -- Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment.
Keywords: Plant science; Botany; Plant Sciences