Receptor Tyrosine Kinases : Methods and Protocols - Germano, Serena; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting -- Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays -- Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Crosslinking -- Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells -- Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved Förster Resonance Energy Transfer (TR-FRET) -- Applying the Proximity Ligation Assay (PLA) to Mouse Preimplantation Embryos for Identifying Protein-Protein Interactions In Situ -- Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1 -- Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalian Cells -- Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking -- Studying N-Linked Glycosylation of Receptor Tyrosine Kinases -- Identification of Receptor Protein Tyrosine Phosphatases (RPTPs) as Regulators of Receptor Tyrosine Kinases (RTKs) Using an RPTP siRNA-RTK Substrate Screen -- Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection -- Regulation of Receptor Tyrosine Kinases by miRNA: Overexpression of miRNA Using Lentiviral Inducible Expression Vectors -- Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases -- Receptor Tyrosine Kinase Targeting in Multicellular Spheroids -- Receptor Tyrosine Kinases and Drug Resistance: Development and Characterization of In Vitro Models of Resistance to RTK Inhibitors.
Keywords: Proteins .; Cell membranes .; Protein Science; Membrane Biology