RNA Nanotechnology and Therapeutics : Methods and Protocols - Guo, Peixuan; Haque, Farzin; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles -- Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly -- Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM -- Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies -- Purification of RNA Nanoparticles by Ultracentrifugation -- HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution -- RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo -- Fluorescence Labeling of Short RNA by Oxidation at the 3'-End -- Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases -- Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor -- Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens -- Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge -- A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells -- Design, Assembly, and Evaluation of RNA-Protein Nanostructures -- Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq -- Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting.
Keywords: Biotechnology; Nanotechnology; Biotechnology; Nanotechnology