Protein Chromatography : Methods and Protocols - Walls, Dermot; Loughran, Sinéad T.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
A Synopsis of Protein and their Purification -- Gel-Filtration Chromatography -- Immunoaffinity Chromatography: Concepts and Applications -- Avoiding Proteolysis During Protein Purification -- Scale-Up of Protein Purification: Downstream Processing Issues -- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources -- Protein Stability: Enhancement and Measurement -- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification -- Storage and Lyophilisation of Pure Proteins -- Differential Precipitation and Solubilization of Proteins -- Ion-Exchange Chromatography: Basic Principles and Application -- Protein Quantitation and Analysis of Purity -- Purification of Proteins Fused to Maltose-Binding Protein -- Purification of Poly-Histidine-Tagged Proteins -- Purification of Antibodies Using Affinity Chromatography -- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens -- Measuring Protein-Protein Interactions Using Biacore -- Hydrophobic Interaction Chromatography. .
Keywords: Proteins .; Protein Science