RNA Interference, Editing, and Modification : Methods and Protocols - Gott, Jonatha M.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2004)
RNA Interference and Gene Silencing -- RNA Interference -- Methods for Delivery of Double-Stranded RNA into Caenorhabditis elegans -- Induction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 Cells -- Analysis of Gene Function in Trypanosoma brucei Using RNA Interference -- Short Hairpin Activated Gene Silencing in Mammalian Cells -- Geminivirus Vectors for Transient Gene Silencing in Plants -- Posttranscriptional Gene Silencing in Plants -- Identification of microRNAs and Other Tiny Noncoding RNAs by cDNA Cloning -- RNA Editing and Modification -- A Historical Perspective on RNA Editing -- Identification of Substrates for Adenosine Deaminases That Act on RNA -- Purification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coli -- Isolation of an mRNA-Binding Protein Involved in C-to-U Editing -- In Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated Activities -- Identification and Characterization of Trypanosome RNA-Editing Complex Components -- Chimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In Vitro -- Methods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellanii -- In Vitro RNA Editing Systems From Higher Plant Chloroplasts -- Studying RNA Editing in Transgenic Chloroplasts of Higher Plants -- Detection and Quantification of Modified Nucleotides in RNA Using Thin-Layer Chromatography -- Functional Characterization of 2?-O-Methylation and Pseudouridylation Guide RNAs -- Experimental Rnomics.
Keywords: Biochemistry; Biochemistry, general