Photosynthesis Research Protocols - Carpentier, Robert; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher Plants -- Isolation of Photosystem I Submembrane Fractions -- Isolation of Photosystem II Reaction Center Complexes from Plants -- Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803 -- Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria -- Isolation of Cytochrome b6f Complex from Grana and Stroma Membranes from Spinach Chloroplasts -- Purification and Crystallization of the Cytochrome b6f Complex -- Purification of Plastocyanin and Cytochrome c6 from Plants, Green Algae, and Cyanobacteria -- Isolation and Identification of Chloroplast Lipids -- Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants -- Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex -- Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies with Light-Inducible Structural Transitions -- Protein Targeting across and into Chloroplast Membranes -- Proteomic Analysis of Thylakoid Membranes -- Thylakoid Phosphoproteins: Identification of Phosphorylation Sites -- Direct Detection of Free Radicals and Reactive Oxygen Species in Thylakoids -- Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher Plants -- Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure-Function Characterization -- Physical and Chemical Immobilization Methods of Photosynthetic Materials -- Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana -- Expression of Genes in Cyanobacteria: Adaptation of Endogenous Plasmids as Platforms for High-Level Gene Expression in Synechococcus sp. PCC 7002 -- Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803 -- A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii -- Rapid Isolation of Intact Chloroplasts from Spinach Leaves -- Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses -- Isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Leaves -- Quantifying the Amount and Activity of Rubisco in Leaves -- Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli -- Rubisco Activase Activity Assays -- Quantification of Rubisco Activase Content in Leaf Extracts.
Keywords: Plant science; Botany; Plant biochemistry; Plant Sciences; Plant Biochemistry