Progranulin : Methods and Protocols - Bateman, Andrew; Bennett, Hugh P.J.; Cheung, Siu Tim.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
A Brief Overview of Progranulin in Health and Disease -- Chromatographic Methods for the Purification of Granulin Peptides -- Methods for Expression and Purification of Biologically Active Recombinant Progranulin -- Large-Scale Generation of Recombinant Granulin Peptides in E. coli -- Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging -- Data Mining: Applying the AD&FTD Mutation Database to Progranulin -- Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases -- Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker -- Analysis of Progranulin-Mediated Akt and MAPK Activation -- Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models -- Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features -- Methods to Study the Role of Progranulin in the Tumor Microenvironment -- Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo using Caenorhabditis elegans -- The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response -- Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function -- Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice -- Methods to Investigate the Protection Againts Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain -- The Interaction between Progranulin with Sortilin and the Lysosome -- Methods to Study the Role of Progranulin in Preimplanatation Mouse Embryo Development -- Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-Inflammatory Activity of Progranulin in Inflammatory Arthritis -- Methods for Studying the Function of Progranulin in Atherosclerosis using Both Knockout Mice Models and In Vitro Studies -- Methods to Investigate the Roles of Prgranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models.
Keywords: Proteins .; Protein Science