RNA'Protein Interaction Protocols - Haynes, Susan R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 1999)
Labeling and Purification of RNA Synthesized by In Vitro Transcription -- Joining RNA Molecules with T4 DNA Ligase -- RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs -- The Methylene Blue Mediated Photocrosslinking Method for Dectection of Proteins that Interact with Double-Stranded RNA -- Probing RNA-Protein Interactions by Psoralen Photocrosslinking -- Identification and Sequence Analysis of RNA-Protein Contact Sites by N-Terminal Sequencing and MALDI-MS -- RNA Footprinting and Modification Interference Analysis -- Oligonucleotide-Targeted RNase H Protection Analysis of RNA-Protein Complexes -- Nitrocellulose Filter Binding for Determination of Dissociation Constants -- Measuring Equilibrium and Kinetic Constants Using Gel Retardation Assays -- PACE Analysis of RNA-Peptide Interactions -- Detection of Nucleic Acid Interactions Using Surface Plasmon Resonance -- An Escherichia coli-Based Genetic Strategy for Characterizing RNA Binding Proteins -- Screening RNA-Binding Libraries Using a Bacterial Transcription Antitermination Assay -- In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display -- In Vitro Selection of Aptamers from RNA Libraries -- Identification of Specific Protein-RNA Target Sites Using Libraries of Natural Sequences -- Northwestern Screening of Expression Libraries -- Screening Expression Libraries with Solution-Based Assay -- An Immunoprecipitation-RNA:rPCR Method for the In Vivo Isolation of Ribonucleoprotein Complexes -- Purification and Depletion of RNP Particles by Antisense Affinity Chromatography -- Purification of U Small Nuclear Ribonucleoprotein Particles -- Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes -- Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing -- Mammalian In Vitro Splicing Assays -- Yeast Pre-mRNA Splicing Extracts -- Prespliceosome and Spliceosome Isolation and Analysis -- A Yeast Spliceosome Assay -- Defining Pre-mRNA cis Elements that Regulate Cell-Specific Splicing -- In Vivo SELEX in Vertebrate Cells -- Purification of SR Protein Splicing Factors -- Processing mRNA 3' Ends In Vitro -- Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay -- In Vitro Translation Extracts from Tissue Culture Cells -- Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes.
Keywords: Cell biology; Cell Biology