Plant Functional Genomics : Methods and Protocols - Alonso, Jose M.; Stepanova, Anna N.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Epigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq -- Whole-Genome DNA Methylation Profiling with Nucleotide Resolution -- High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes -- Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants -- Characterization of in-vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis -- Identification of Direct Targets of Plant Transcription Factors Using the GR Fusion Technique -- Ribosome Profiling: A Tool for Quantitative Evaluation of Dynamics in mRNA Translation -- Tissue-Specific Gene Expression Profiling by Cell Sorting -- Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes -- Rapid Immunopurification of Ribonucleoprotein Complexes of Plants -- Metabolomic Profiling of Plant Tissues -- Targeted Plant Genome Editing via the CRISPR/Cas9 Technology -- QTL Mapping Using High-Throughput Sequencing -- Quantitating Plant MicroRNA-Mediated Target Repression Using a Dual-Luciferase Transient Expression System -- Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis -- A User's Guide to the Arabidopsis T-DNA Insertion Mutant Collections -- Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana -- Tilling By Sequencing -- SHOREmap v3.0: Fast and Accurate Identification of Causal Mutations from Forward Genetic Screens -- Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework -- Ligation Independent Cloning for Plant Research -- Gene Functional Analysis Using Protoplast Transient Assays -- Descriptive vs. Mechanistic Network Models in Plant Development in the Post-Genomic Era -- Analysis and Visualization of RNA-Seq Expression Data Using RStudio, Bioconductor, and Integrated Genome Browser -- Constructing Simple Biological Networks for Understanding Complex High-Throughput Data in Plants.
Keywords: Plant science; Botany; Plant Sciences