Polycomb Group Proteins : Methods and Protocols - Lanzuolo, Chiara; Bodega, Beatrice; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Mapping the Function of Polycomb Proteins -- Chromatin Immunoprecipitation -- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos -- ChIP-seq data processing for PcG proteins and associated histone modifications -- Analysis of single locus replication timing in asynchronous cycling cells -- Non coding RNA interplay with the genome -- RIP: RNA Immunoprecipitation -- Capture Hybridization Analysis of DNA Targets -- Identification of RNA-protein interactions through in-vitro RNA pull-down assays -- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP) -- Analysis RNA-seq and non coding RNA -- The dynamics of Polycomb complexes -- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics -- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells -- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure -- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images -- Polymer physics of the large-scale structure of chromatin -- Chromosome Conformation Capture in Drosophila -- Chromosome Conformation Capture in primary human cells -- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq) -- Chromosome Conformation Capture on Chip (4C): Data Processing -- In vivo models to address the function of Polycomb Group Proteins -- A rapid TALEN assembly protocol -- Following the motion of Polycomb bodies in living Drosophila embryos -- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion -- Imaginal discs transplantation in Drosophila -- Isolation and culture of muscle stem cells. .
Keywords: Proteins .; Protein Science