Patch-Clamp Methods and Protocols - Martina, Marzia; Taverna, Stefano; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2014)
History of Electrophysiology and the Patch-Clamp.- Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications -- Patch-Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-methyl-D-aspartate (NMDA) Receptor Open Channel Blockers -- State-of-the-art Automated Patch-Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening -- Industrializing Electrophysiology: HT Automated Patch-Clamp on SyncroPatch® 96 Using Instant Frozen Cells -- Planar Patch Clamp for Neuronal Networks-Considerations and Future Perspectives -- Methods for the Study of Synaptic Receptor Functional Properties -- Single-Cell RT-PCR, A Technique to Decipher the Electrical, Anatomical and Genetic Determinants of Neuronal Diversity -- Whole-Cell Voltage-Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique -- Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability -- Investigation of Synaptic Microcircuits Using Paired Patch-Clamp Recordings in Acute Brain Slices -- Outside-Out 'Sniffer-patch' Clamp Technique for in situ Measures of Neurotransmitter Release -- A Cost Effective Method for Preparing, Maintaining and Transfecting Neurons in Organotypic Slices -- Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics -- Synaptic Connectivity in Engineered Neuronal Networks -- Modeling of Action Potential Generation in NG108-15 Cells -- Whole-Cell Patch-Clamp Recordings in Freely Moving Animals -- Pressure-Polished Borosilicate Pipettes are "Universal Sealer" Yielding Low Access Resistance and Efficient Intracellular Perfusion -- Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes -- Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents -- Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI -- Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes -- Principles of Single-Channel Kinetic Analysis.
Keywords: Neurosciences; Human physiology; Neurosciences; Human Physiology