Plant Secondary Metabolism Engineering : Methods and Applications - Fett-Neto, Arthur Germano; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview -- Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin -- Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System -- Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis -- Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid -- Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength -- Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity -- Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties -- Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates -- Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture -- of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination -- Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids -- USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories -- Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene -- Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants -- Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes -- Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering -- Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.) -- Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis -- Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries -- Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters.
Keywords: Biotechnology; Plant science; Botany; Plant genetics; Gene expression; Biotechnology; Plant Sciences; Plant Genetics and Genomics; Gene Expression