Plant Receptor Kinases : Methods and Protocols - Aalen, Reidunn Birgitta; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Expression of Plant Receptor Kinases in E. coli -- Expression of Plant Receptor Kinases in Yeast -- Expression of Plant Receptor Kinases in Tobacco BY-2 Cells -- Cell-free Synthesis of Plant Receptor Kinases -- Purification of Plant Receptor Kinases from Plant Plasma Membranes -- Photoaffinity Labeling of Plant Receptor Kinases -- Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases -- Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis -- In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping -- Co-immunoprecipitation of Plant Receptor Kinases -- Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems -- Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners -- In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases -- BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases -- Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases -- The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases -- Detection and Analyses of Endocytosis of Plant Receptor Kinases.
Keywords: Plant science; Botany; Plant Sciences