Plant Lipid Signaling Protocols - Munnik, Teun.; Heilmann, Ingo.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC -- Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC -- Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography -- Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay -- Quantification of Diacylglycerol by Mass Spectrometry -- Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK -- Use of Phospholipase A2 for the Production of Lysophospholipids -- Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography -- Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry -- Detection and Quantification of Plant Sphingolipids by LC-MS -- Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid -- Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants -- Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR -- Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides -- Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy -- kinase Assays -- Assaying Inositol and Phosphoinositide Phosphatase Enzymes -- Determination of Phospholipase C Activity In Vitro -- Assaying Non-Specific Phospholipase C (NPC) Activity -- Assaying Different Types of Plant Phospholipase D Activities In Vitro -- Measuring PLD Activity In Vivo -- Assaying Plant Phosphatidic Acid Phosphatase Activity -- Assay of Phospholipase A Activity -- Lipid-Binding Analysis using a Fat Blot Assay -- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions -- Lipid affinity beads - From Identifying New Lipid Binding Proteins to Assessing their Binding Properties -- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants -- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy.
Keywords: Plant science; Botany; Plant Sciences