Phytoplasma : Methods and Protocols - Dickinson, Matt.; Hodgetts, Jennifer; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
The Phytoplasmas - An Introduction -- Techniques for the Maintenance and Propagation of Phytoplasmas in Glasshouse Collections of Catharanthus roseus -- Micropropagation and Maintenance of Phytoplasmas in Tissue Culture -- Dodder Transmission of Phytoplasmas -- Insect Maintenance and Transmission -- Capturing Insect Vectors of Phytoplasmas -- Insect Vector Transmission Assays -- Molecular Identification of Phytoplasma Vector Species -- Dienes' Staining and Light Microscopy for Phytoplasma Visualization -- DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas -- Visualisation of Phytoplasmas using Electron Microscopy -- Automated DNA Extraction for Large Numbers of Plant Samples -- DNA Extraction from Arborescent Monocots and How To Deal With Other Challenging Hosts -- Nested PCR and RFLP Analysis Based on the 16S rRNA Gene -- PCR and RFLP Analyses Based on the Ribosomal Protein Operon -- Tuf and secY PCR Amplification and Genotyping of Phytoplasmas -- PCR Analysis of Phytoplasmas Based on the secA Gene -- Single-Strand Conformation Polymorphism Analysis for Differentiating Phytoplasma Strains -- Microarrays for Universal Detection and Identification of Phytoplasmas -- T-RFLP for Detection and Identification of Phytoplasmas in Plants -- Real-Time PCR for Universal Phytoplasma Detection and Quantification -- A Real-Time PCR Detection System for the Bois Noir and Flavescence Dorée Phytoplasmas and Quantification of the Target DNA -- Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group -- Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions -- In-Field Diagnostics Using Loop-Mediated Isothermal Amplification (LAMP) -- DNA Bar-Coding for Phytoplasma Identification -- Phylogenetic Analyses of Phytoplasmas Based on Whole-Genome Comparison -- The iPhyClassifier, An Interactive Online Tool for Phytoplasma Classification and Taxonomic Assignment -- Phytoplasma Proteomic Analysis -- Preparation of Phytoplasma Membrane Recombinant Proteins -- Phytoplasma Plasmid DNA Extraction -- Caesium Chloride-Bisbenzimide Gradients for Separation of Phytoplasma and Plant DNA -- Pulsed-Field Gel Elecrophoresis for Isolation of Full-Length Phytoplasma Chromosomes From Plants -- Mapping the Phytoplasma Chromosome.
Keywords: Plant science; Botany; Plant genetics; Plant Sciences; Plant Genetics and Genomics