Nucleic Acid Crystallography : Methods and Protocols - Ennifar, Eric.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Perspectives and Pitfalls in Nucleic Acids Crystallography -- Preparation of Short 5'-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies -- Preparation and Crystallization of Riboswitches -- In Vitro/In Vivo Production of tRNA for X-Ray Studies -- Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA -- Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs -- Fab Chaperone-Assisted RNA Crystallography (Fab CARC) -- Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes -- Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes -- Crystallographic Data and Model Quality -- Advanced Crystallographic Data Collection Protocols for Experimental Phasing -- Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases -- Practical Radiation Damage Induced Phasing -- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data -- Using Molecular Replacement Phasing to Study the Structure and Function of RNA -- Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution? -- RNA Structure Refinement Using the ERRASER-Phenix Pipeline -- Neutron Nucleic Acid Crystallography -- Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins -- Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers -- Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor -- Anions in Nucleic Acid Crystallography.
Keywords: Nucleic acids; Nucleic Acid Chemistry