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100715s2010 xxu| s |||| 0|eng d |
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|a 9781603273893
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| 024 |
7 |
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|a 10.1007/978-1-60327-389-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Rat Genomics :
|b Methods and Protocols /
|c edited by Ignacio Anegon.
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| 250 |
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|a 1st ed. 2010.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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| 300 |
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|a XVI, 462 p. 70 illus., 2 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 597
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| 505 |
0 |
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|a The Rat: A Model Used in Biomedical Research -- Genetic Mapping and Positional Cloning -- Sequencing of the Rat Genome and Databases -- Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators) -- Inducible and Conditional Promoter Systems to Generate Transgenic Animals -- Generation of Transgenic Rats by Microinjection of Short DNA Fragments -- Generation of Transgenic Rats Using YAC and BAC DNA Constructs -- The Use of Lentiviral Vectors to Obtain Transgenic Rats -- Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA -- Procedures for Somatic Cell Nuclear Transfer in the Rat -- ENU Mutagenesis to Generate Genetically Modified Rat Models -- Establishment of Embryonic Stem Cells from Rat Blastocysts -- Derivation, Culture, and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts -- Interference RNA for In vivo Knock-Down of Gene Expression or Genome-Wide Screening Using shRNA -- Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases -- Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics -- Generation of Congenic and Consomic Rat Strains -- Generation of Rat “Supersonic” Congenic/Conplastic Strains Using Superovulation and Embryo Transfer -- Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms -- A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites -- Cryopreservation and Orthotopic Transplantation of Rat Ovaries -- Techniques for In Vitro and In Vivo Fertilization in the Rat -- Rat Strain Repositories -- Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases -- Rat Genomics Applied to Psychiatric Research -- Genomics Studies of Immune-Mediated Diseases Using the BN–LEW Rat Model -- Rat Models of Cardiovascular Diseases -- Use of Rat Genomics for Investigating the Metabolic Syndrome -- Genomic Research in Rat Models of Kidney Disease -- Cancer Research in Rat Models.
|
| 650 |
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0 |
|a Biochemistry.
|
| 650 |
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0 |
|a Zoology.
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| 650 |
|
0 |
|a Human physiology.
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| 650 |
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0 |
|a Animal genetics.
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| 650 |
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0 |
|a Transgenic organisms.
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| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 650 |
2 |
4 |
|a Zoology.
|
| 650 |
2 |
4 |
|a Human Physiology.
|
| 650 |
2 |
4 |
|a Animal Genetics and Genomics.
|
| 650 |
2 |
4 |
|a Transgenics.
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| 700 |
1 |
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|a Anegon, Ignacio.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|