Rat Genomics : Methods and Protocols - Anegon, Ignacio; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
The Rat: A Model Used in Biomedical Research -- Genetic Mapping and Positional Cloning -- Sequencing of the Rat Genome and Databases -- Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators) -- Inducible and Conditional Promoter Systems to Generate Transgenic Animals -- Generation of Transgenic Rats by Microinjection of Short DNA Fragments -- Generation of Transgenic Rats Using YAC and BAC DNA Constructs -- The Use of Lentiviral Vectors to Obtain Transgenic Rats -- Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA -- Procedures for Somatic Cell Nuclear Transfer in the Rat -- ENU Mutagenesis to Generate Genetically Modified Rat Models -- Establishment of Embryonic Stem Cells from Rat Blastocysts -- Derivation, Culture, and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts -- Interference RNA for In vivo Knock-Down of Gene Expression or Genome-Wide Screening Using shRNA -- Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases -- Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics -- Generation of Congenic and Consomic Rat Strains -- Generation of Rat "Supersonic" Congenic/Conplastic Strains Using Superovulation and Embryo Transfer -- Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms -- A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites -- Cryopreservation and Orthotopic Transplantation of Rat Ovaries -- Techniques for In Vitro and In Vivo Fertilization in the Rat -- Rat Strain Repositories -- Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases -- Rat Genomics Applied to Psychiatric Research -- Genomics Studies of Immune-Mediated Diseases Using the BN-LEW Rat Model -- Rat Models of Cardiovascular Diseases -- Use of Rat Genomics for Investigating the Metabolic Syndrome -- Genomic Research in Rat Models of Kidney Disease -- Cancer Research in Rat Models.
Keywords: Biochemistry; Zoology; Human physiology; Animal genetics; Transgenic organisms; Biochemistry, general; Zoology; Human Physiology; Animal Genetics and Genomics; Transgenics