Plant Fungal Pathogens : Methods and Protocols - Bolton, Melvin D.; Thomma, Bart P.H.J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2012)
Fungal ABC Transporter Deletion and Localization Analysis -- Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation -- Activity-based Protein Profiling of Infected Plants -- The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves -- Proteomic Techniques for Plant-Fungal Interactions -- Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector -- Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens -- Real-time PCR for the Quantification of Fungi in planta -- Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi -- Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates -- Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors -- Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens -- Heterologous Production of Fungal Effectors in Pichia pastoris -- The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta -- Metabolomics Protocols for Filamentous Fungi -- Targeted Gene Replacement in Fungi Using a Split-Marker Approach -- Massively Parallel Sequencing Technology in Pathogenic Microbes -- Confocal Microscopy in Plant Pathogen Interactions -- The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data -- Population Biology of Fungal Plant Pathogens -- Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens -- In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi -- Fungicide Resistance Assays for Fungal Plant Pathogens -- Identification of Lipid-binding Effectors -- In silico Identification and Characterization of Effector Catalogs -- Horizontal Transfer of Supernumerary Chromosomes in Fungi -- The Induction of Mycotoxins by Trichothecene Producing Fusarium Species -- Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics -- Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM) -- Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays -- Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors -- A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts -- Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes -- Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens -- MAP Kinase Phosphorylation and cAMP Assessment in Fungi -- A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens -- Karyotyping Methods for Fungi -- Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique -- Gene Cloning using Degenerate Primers and Genome Walking -- Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi -- An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ.
Keywords: Plant science; Botany; Plant pathology; Plant Sciences; Plant Pathology