Neutrophil Methods and Protocols - Quinn, Mark T.; DeLeo, Frank R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
The Role of Neutrophils in the Immune System: An Overview -- Isolation of Human Neutrophils -- Neutrophil Isolation from Non-Human Species -- Collection of In vivo Transmigrated Neutrophils from Human Skin -- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers -- Rho Family and Rap GTPase Activation Assays -- Measurement of Phospholipid Metabolism in Intact Neutrophils -- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils -- Analysis of Electrophysiological Properties and Responses of Neutrophils -- Analysis of Neutrophil Apoptosis -- Microinjection Methods for Neutrophils -- Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells -- Neutrophil Migration Through Extracellular Matrix -- Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish -- Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion -- Immunofluorescence and Confocal Microscopy of Neutrophils -- Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils -- Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry -- Analysis of Neutrophil Bactericidal Activity -- Induction and Quantification of Neutrophil Extracellular Traps -- Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes -- Cell-free NADPH Oxidase Assays: "In vitro Veritas" -- Assessment of Priming of the Human Neutrophil Respiratory Burst -- Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane -- Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies -- Genome-Scale Transcript Analyses with Human Neutrophils -- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils -- High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis -- Detection of Intact Transcription Factors in Human Neutrophils -- Disorders of Neutrophil Function: An Overview -- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders -- Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency.
Keywords: Infectious diseases; Microbial genetics; Microbial genomics; Infectious Diseases; Microbial Genetics and Genomics