Polyadenylation : Methods and Protocols - Rorbach, Joanna; Bobrowicz, Agnieszka J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2014)
3' end Formation and Regulation of Eukaryotic mRNAs -- Poly(A) Polymerase-based Poly(A) Length assay -- Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome -- Detection of Uridylated mRNAs -- Cytoplasmic Polyadenylation Assays -- In vitro Analysis of Transcriptional Activators and Polyadenylation -- Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2 -- Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity -- Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing -- Polyadenylation Site Prediction using PolyA-iEP Method -- Detecting alternative polyadenylation from Microarray Data -- Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing -- RHAPA: A New Method to Quantify Alternative Polyadenylation -- Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA -- Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq -- High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs -- Polyadenylation in Bacteria and Organelles -- In vivo Analysis of Polyadenylation in Prokaryotes -- Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities -- Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends -- Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine -- Deadenylation and its Regulation in Eukaryotic Cells -- Assaying mRNA Deadenylation in vitro -- Assaying mRNA Deadenylation in vivo -- In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes -- microRNA-mediated Deadenylation in a Mammalian Cell-free System -- Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay.
Keywords: Human genetics; Medical genetics; Human Genetics; Gene Function