Neurotrophic Factors : Methods and Protocols - Skaper, Stephen D.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
1. Neurotrophic Factors: An Overview.- Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions -- Culture of Rodent Cortical, Hippocampal, and Striatal Neurons -- Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum -- Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents -- Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays -- Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening -- Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents -- Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice -- Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation -- Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity -- Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair -- Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia -- Diolistic Labeling and Analysis of Dendritic Spines -- Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses -- Methods for Quantitative Analysis of Axonal Cargo Transport -- Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis -- Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay -- Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay.- Rat Pancreatic Beta-Cell Culture -- Growth and Neurotrophic Factors in Embryonic Stem Cells -- Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications -- Isolation and Culture of Brain Microvascular Endothelial Cells for In Vitro Blood-Brain Barrier Studies -- Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity -- An Animal Model of Alzheimer Disease Based on the Intrahippocampal Injection of Amyloid β-Peptide (1-42) -- Active Induction of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice -- A Model of Systemic Inflammation to Study Neuroinflammation -- Spared Nerve Injury as a Long-Lasting Model of Neuropathic Pain -- An In Vivo Compression Model of Spinal Cord Injury -- A Controlled Cortical Impact Preclinical Model of Traumatic Brain Injury -- Middle Cerebral Artery Occlusion by an Intraluminal Suture Method -- Primary Motor Neuron Culture to Promote Cellular Viability and Myelination -- Culture of Human Primary Bone Cells and Phenotype Assessment -- Recreating Intestinal Peristalsis in the Petri Dish -- Synthesis of Biocompatible Titanate Nanofibers for Effective Delivery of Neuroprotective Agents -- Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides.
Keywords: Neurosciences; Neurosciences