Proteomic Profiling : Methods and Protocols - Posch, Anton; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization -- Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples -- Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting -- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling -- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model -- Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions -- A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain -- Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses -- Dynamic Range Compression with ProteoMiner: Principles and Examples -- Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue -- Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics -- Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry -- P Isolation of Extracellular Vesicles for Proteomic Profiling -- A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods -- Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes -- Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method -- Step by Step Preparation of Proteins for Mass Spectrometric Analysis -- Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry -- Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS -- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation -- Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing -- Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis -- Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation -- Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry -- In-Gel Peptide IEF Sample Preparation for LC/MS Analysis -- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology -- 2-D Western Blotting for Evaluation of Antibodies Developed for Detection of Host Cell Protein -- Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes -- Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes -- A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin -- Identification of SUMO E3 Ligase Specific Substrates Using the HuProt Human Proteome Microarray -- Amyloid-Binding Proteins: Affinity Based Separation, Proteomic Identification and Optical Biosensor Validation -- Proteomic Profiling by Nanomaterials-Based Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for High-Resolution Data and Novel Protein Information Directly from Biological Samples.
Keywords: Proteins .; Proteomics; Protein Science; Proteomics