Plant Germline Development : Methods and Protocols - Schmidt, Anja.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
An Introduction to Male Germline Development -- Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants -- 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice -- Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana -- Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers -- Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy -- In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes -- Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins -- Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes -- Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy -- Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures -- Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues -- Whole Mount RNA-FISH on Ovules and Developing Seeds -- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy -- Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes -- Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS) -- Isolation of Rice Sperm Cells for Transcriptional Profiling -- Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation -- Isolation and Detection of Small RNAs from Pollen -- Analysis of Proteins Enriched in Rice Gamete -- Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract -- Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants -- RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis -- RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data -- Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality -- Agrobacterium-Mediated Sorghum Transformation -- Use of the Cas9 Orthologues from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana -- Transgenic Reproductive Cell Ablation -- In Vivo Reporters for Protein Half-Life -- Conditional Modulation of Biological Processes by Low-Temperature Degrons.
Keywords: Plant science; Botany; Plant Sciences