Receptor Signal Transduction Protocols : Third Edition - Willars, Gary B.; Challiss, R.A. John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Inducible Expression of G Protein-Coupled Receptors in Transfected Cells -- Using the Flp-InTM T-RexTM System to Regulate GPCR Expression -- Viral Infection for GPCR Expression in Eukaryotic Cells -- Generation of Epitope-Tagged GPCRs -- The Use of Site-Directed Mutagenesis to Study GPCRs -- Approaches to Study GPCR Regulation in Native Systems -- Heterologous Expression of GPCRs in Fission Yeast -- Radioligand Binding Methods for Membrane Preparations and Intact Cells -- Quantification of GPCR mRNA Using Real-Time RT-PCR -- Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data -- Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells -- Examining Site-Specific GPCR Phosphorylation -- Ubiquitination of GPCRs -- [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs -- Using Calcium Imaging as a Readout of GPCR Activation -- Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors -- Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins -- The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events -- Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Study of GPCR-Protein Interactions by BRET -- Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization -- Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes -- Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes -- Identification of GPCR Localization in Detergent Resistant Membranes -- Analysis of GPCR Localization and Trafficking -- Statistical Methods in Research.
Keywords: Biochemistry; Biochemistry, general