RNA Detection : Methods and Protocols - Gaspar, Imre.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
The Secret Life of RNA: Lessons from Emerging Methodologies -- Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol) -- Identifying the m6A Methylome by Affinity Purification and Sequencing -- PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution -- Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo -- LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing -- Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas -- Single mRNA Molecule Detection in Drosophila -- Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos -- Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction -- Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections -- Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification -- In Situ Detection of MicroRNA Expression with RNAscope Probes -- Padlock Probes to Detect Single Nucleotide Polymorphisms -- Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons -- Optimizing Molecular Beacons for Intracellular Analysis of RNA -- Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH -- In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes -- Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers -- Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe -- RNA Live Imaging in the Model Microorganism Ustilago maydis -- Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells -- Live Imaging of mRNA Synthesis in Drosophila -- Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog -- Detection of the First Round of Translation: The TRICK Assay -- Imaging Translation Dynamics of Single mRNA Molecules in Live Cells -- Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding -- Isolation and Characterization of Endogenous RNPs from Brain Tissues -- Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions -- RNA Tagging: Preparation of High-Throughput Sequencing Libraries -- RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.
Keywords: Human genetics; Human Genetics