Protein-Carbohydrate Interactions : Methods and Protocols - Abbott, D. Wade.; Lammerts van Bueren, Alicia; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases -- Quantitative Kinetic Characterization of Glycoside Hydrolases using High-Performance Anion-Exchange Chromatography (HPAEC) -- Measuring Enzyme Kinetics of Glycoside Hydrolases using the 3,5-Dinitrosalicylic Acid Assay -- An Improved Kinetic Assay for the Characterization of Metal-Dependant Pectate Lyases -- Colorimetric Detection of Acetyl Xylan Esterase Activities -- Methods for Determining Glycosyltransferase Kinetics -- Analyzing Activities of Lytic Polysaccharide Monooxygenases by Liquid Chromatography and Mass Spectrometry -- Carbohydrate Depolymerization by Intricate Cellulosomal Systems -- Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions -- Quantifying CBM Carbohydrate Interactions using Microscale Thermophoresis -- Characterization of Protein-Carbohydrate Interactions by NMR Spectroscopy -- Measuring the Biomechanical Loosening Action of Bacterial Expansins on Paper and Plant Cell Walls -- Bioinspired Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP -- CBMs as Probes to Explore Plant Cell Wall Heterogeneity using Immunocytochemistry -- Determining the Localization of Carbohydrate Active Enzymes within Subcellular Compartments of Gram-Negative Bacteria -- Analysis of Complex Carbohydrate Composition in Plant Cell Wall using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR) -- Separation and Visualization of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis -- A Rapid Procedure for the Purification of 8-Aminopyrene Trisulfonate (APTS)-Labelled Glycans for Capillary Electrophoresis (CE)-Based Enzyme Assays -- Probing the Complex Architecture of Multimodular Carbohydrate-Active Enzymes using a Combination of Small Angle X-Ray Scattering and X-Ray Crystallography -- Metagenomics and CAZyme Discovery -- Identification of Genes Involved in the Degradation of Lignocellulose using Comparative Transcriptomics -- Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies using Mass Spectrometry.
Keywords: Proteins .; Protein Science