Phospho-Proteomics : Methods and Protocols - von Stechow, Louise; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Springer, 2016)
Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins -- Phosphopeptide Detection with Biotin-Labeled Phos-tag -- Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports -- Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics -- Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles -- Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM) -- Enrichment Strategies in Phosphoproteomics -- Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography -- The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides -- Sequential Elution from IMAC (SIMAC) - An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides -- Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation -- Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage -- Phosphopeptide Enrichment Using Various Magnetic Nanocomposites - An Overview -- Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics -- Variable Digestion Strategies for Phosphoproteomics Analysis -- On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins -- Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity -- Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics -- Phosphoprotein Detection by High-Throughput Flow Cytometry -- Resources for Assignment of Phosphorylation Sites on Peptides and Proteins -- From Phosphosites to Kinases -- Search Databases and Statistics - Pitfalls and Best Practices in Phosphoproteomics -- Systems Analysis for Interpretation of Phosphoproteomics Data.
Keywords: Proteins .; Proteomics; Protein Science; Proteomics