Protein Tyrosine Phosphatases : Methods and Protocols - Pulido, Rafael; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
The Extended Family of Protein Tyrosine Phosphatases -- Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome -- Expression, Purification, and Kinetic Analysis of PTP Domains -- Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples -- Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B -- Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates -- Assessing the Biological Activity of the Glucan Phosphatase Laforin -- Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents -- Analyzing Pseudophosphatase Function -- Crystallization of PTP Domains -- NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases -- Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs) -- In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions -- Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates -- Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains -- Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling -- Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis -- Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis -- Studying Protein-Tyrosine Phosphatases in Zebrafish -- Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands -- Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs.
Keywords: Proteins .; Protein Science