NLR Proteins : Methods and Protocols - Di Virgilio, Francesco; Pelegrín, Pablo; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Innate Immune Receptors -- Atypical Inflammasomes -- Assessment of Inflammasome Activation By Cytokine And Danger Signal Detection -- Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging -- Measuring IL-1β Processing By Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor -- Measuring IL-1β Processing By Bioluminescence Sensors II: The Igluc System -- Assessing Extracellular ATP as Danger Signal In Vivo: The Pmeluc System -- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-Immunoprecipitation and Cross-Linking -- Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence -- Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer -- Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity Of Inflammasome Components -- Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay -- Assessing Caspase-1 Activation -- Cell-Free Assay for Inflammasome Activation -- Functional Reconstruction of Nlrs In HEK293 Cells -- Method to Measure Ubiquitinization of Nlrs -- Cytofluorometric Quantification of Cell Death Elicited By NLR Proteins -- NLR in Human Diseases: Role and Laboratory Findings.
Keywords: Proteins .; Protein Science