Prions : Methods and Protocols - Lawson, Victoria A.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Purification and Fibrillation of Full-length Recombinant PrP -- Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure -- Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy -- Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy -- Immunodetection of PrPSc using Western Immunoblotting Techniques -- Analysis of miRNA Signatures in Neurodegenerative Prion Disease -- Expression of Heterologous PrP and Prion Propagation in RK13 Cells -- Generation of Infectious Prions and Detection with the Prion Infected Cell Assay -- Analysis of Cellular Prion Protein Endoproteolytic Processing -- Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology -- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons -- Methods of Protein Misfolding Cyclic Amplification -- RT-QuIC Assays for Prion Disease Detection and Diagnostics -- A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein -- Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases.- In vivo Near Infrared Imaging of Neurodegeneration -- Strain Typing of Prion Diseases using in vivo Mouse Models -- Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice -- Cell Culture Methods for Screening of Prion Therapeutics -- Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease -- Methods for Molecular Diagnosis of Human Prion Disease -- Molecular sub-typing of PrPres in Human Sporadic CJD Brain Tissue -- Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture.
Keywords: Neurosciences; Neurosciences