Tumor Profiling : Methods and Protocols - Murray, Sarah S.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Amplicon-based Targeted Next Generation Sequencing of Formalin Fixed Paraffin Embedded Tissue -- Library preparation using FFPE-derived Tumor DNA for High Throughput Hybridization-based Targeted or Exome Sequencing -- Bioinformatics Basics for High Throughput Hybridization-based Targeted DNA Sequencing From FFPE-Derived Tumor Specimens: From Reads to Variants -- Annotation of Variant Data from High Throughput DNA Sequencing from Tumor Specimens: Filtering Strategies to Identify Driver Mutations -- Biological Interpretation of Complex Genomic Data -- Clinical Validation of Targeted Solid Tumor Profiling -- Whole Genome Single Nucleotide Polymorphism Microarray for Copy Number and Loss of Heterozygosity Analysis in Tumors -- Genome-Wide Copy Number Variation Detection using NGS: Data Analysis and Interpretation -- Overview of Fusion Detection Strategies Using Next Generation Sequencing -- Clinical Application of Fusion Gene Detection Using Next-generation Sequencing and the NanoString Technology -- Pipeline for Integrated Microarray Expression Normalization Toolkit (PIMENTo) for Tumor Microarray Profiling Experiment -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: Overview of Library Preparation Methods -- Molecular Profiling of RNA Tumors using High Throughput RNA Sequencing: From Raw Data to Systems Level Analyses -- Methylation Analysis using Microarrays: Analysis and Interpretation -- High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) -- A Pipeline for ctDNA Detection Following Primary Tumor Profiling Using A Cancer-related Gene Sequencing Panel -- Single-cell Analysis of Circulating Tumor Cells.
Keywords: Molecular biology; Cancer research; Molecular Medicine; Cancer Research