Transgenic Mouse : Methods and Protocols - Larson, Melissa A.; SpringerLink (Online service) (New York, NY : Springer US : Imprint: Humana, 2020)
The History of Transgenesis -- Pronuclear Microinjection of One-Cell Embryos -- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection -- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos -- Targeted Mutations in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device -- Nuclear Transfer and Cloning -- Sperm-Mediated Genetic Modifications -- Genotyping Genetically Modified (GM) Mice -- Nomenclature: Naming Your Gene-Modified Mouse -- Breeding Strategies for Genetically Modified Mice -- Strategies for Behaviorally Phenotyping the Transgenic Mouse -- Cryobanking and Recovery of Genetically Modified Mice -- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques -- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.
Keywords: Human genetics; Animal models in research; Stem cells; Human Genetics; Animal Models; Stem Cells