Techniques in Molecular Biology /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Dordrecht :
Springer Netherlands : Imprint: Springer,
1983.
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| Edición: | 1st ed. 1983. |
| Materias: |
Tabla de Contenidos:
- 1. High Performance Liquid Chromatography of Proteins and Nucleic Acids
- 2. Analytical Electrophoretic Techniques in Protein Chemistry
- 3. Protein Blotting
- 4. The Production and Isolation of Peptides for Sequence Analysis
- 5. Protein and Peptide Sequence Determination
- 6. The Extraction and Fractionation of RNA
- 7. Protein Synthesis In Vitro
- 8. Labelling DNA In Vitro - Nick Translation
- 9. Synthesis of cDNA for Molecular Cloning
- 10. Plasmid Isolation
- 11. The Use of Restriction Endonucleases and T4 DNA Ligase
- 12. Molecular Cloning of cDNA: Bacterial Transformation and Screening of Transformants
- 13. The Use of Minicells and Maxicells to Detect the Expression of Cloned Genes
- 14. Gel Electrophoresis of DNA
- 15. Detection of Specific DNA Sequences - The Southern Blot
- 16. The Determination of DNA Sequences
- 17. The Use of Cosmids as Cloning Vehicles
- Notes on Contributors.