The Surfaceome : Methods and Protocols - Boheler, Kenneth R.; Gundry, Rebekah L.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens -- Shaving" Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins -- Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes.- Mass Spectrometry Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and their Derivatives.- Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and its Proteome from Cells in Suspension.- Proteomic Profiling of Secreted Proteins, Exosomes, and Microvesicles in Cell Culture Conditioned Media.- Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-phosphate Receptor, from Sf9 Cells Using the Baculovirus System.- Bispecific Antibody Armed T Cells to Target Cancer Cells -- Immunophenotyping of Live Human Pluripotent Stem Cells by Flow Cytometry.- Detecting Cell Surface Expression of the G Protein-Coupled Receptor CXCR4.- NaV Channels: Assaying Biosynthesis, Trafficking, Function.- High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs).- Methods for Evaluation of Vascular Endothelial Cell Function with Transient Receptor Potential (TRP) Channel Drugs.- Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase TrkB in Neurons -- Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.- Extracellular Matrix Molecule-Based Capture of Mesenchymal Stromal Cells under Flow.- Generation of Induced Pluripotent Stem Cells from Patients with COL3A1 Mutations and Differentiation to Smooth Muscle Cells for ECM-Surfaceome Analyses -- Fabrication and Mechanical Properties Measurements of 3D Microtissues for the Study of Cell-Matrix Interactions.- Discovery of Surface Target Proteins Linking Drugs, Molecular Markers, Gene Regulation, Protein Networks, and Disease by Using a Web-Based Platform Targets-Search.
Keywords: Cell biology; Cell Biology