Systems Chemical Biology : Methods and Protocols - Ziegler, Slava; Waldmann, Herbert; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
1. PREDECT protocols for complex 2D/3D cultures -- 2. Phenotypic Screening using Mouse and Human Stem Cell-based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses -- 3. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer -- 4. Target Engagement of Small Molecules - Thermal Profiling Approaches on Different Levels -- 5. Visualizing Bio-active Small Molecules by Alkyne-tagging and Slit-scanning Raman Microscopy -- 6. The Cell Painting Assay as a screening tool for the discovery of bioactivities in new chemical matter -- 7. Proteomic Profiling for Target Identification of Biologically Active Small Molecules using 2-D DIGE -- 8. Examining Cellular Responses to Kinase Drug Inhibition through Phosphoproteome Mapping of Substrates -- 9. CRISPR/Cas9-based Chemogenomic Profiling in Mammalian Cells -- 10. Exome Sequencing of Drug-resistant Clones for Target Identification -- 11. The use of large-scale chemically-induced transcriptome data acquired from LINCS to study small molecules -- 12. Integrated Analysis of Drug Sensitivity and Selectivity to Predict Synergistic Drug Combinations and Target Co-addictions in Cancer -- 13. Predicting Drug Interactions from Chemogenomics using INDIGO -- 14. Computational Analyses Connect Small-Molecule Sensitivity to Cellular Features Using Large Panels of Cancer Cell Lines -- 15. Web-based tools for Polypharmacology Prediction -- 16. In silico Target Prediction for Small Molecules.
Keywords: Bioinformatics; Bioinformatics