The Hippo Pathway : Methods and Protocols - Hergovich, Alexander; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
The Power of Drosophila Genetics: The Discovery of the Hippo Pathway -- Drosophila Genetics: The Power of Genetic Mosaic Approaches -- Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues -- Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs -- Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence -- Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions -- Immunohistochemistry to Study YAP in Human Tissue Samples.-Immunofluorescence Study of Endogenous YAP in Mammalian Cells -- Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells -- Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors -- Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells -- Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells -- HTRF® Total and Phospho-YAP(Ser127) Cellular Assays -- Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids -- Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview -- CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels -- Hippo Pathway Regulation by Tyrosine Kinases -- Structural and Biochemical Analyses of the Core Components of the Hippo Pathway -- Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro -- GST Pull-Down Assay to Measure Complex Formations -- Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag -- Quantifying the Kinase Activities of MST1/2 -- Measuring the Kinase Activities of the LATS/NDR Protein Kinases -- MST1/2 Kinase Assays Using Recombinant Proteins -- Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development -- The Hippo Signaling Pathway in Regenerative Medicine.
Keywords: Biochemistry; Biochemistry, general