Skin Tissue Engineering : Methods and Protocols - Böttcher-Haberzeth, Sophie; Biedermann, Thomas; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Part 1 : Isolation and culture of skin cells -- Chap 1: Isolation and Culture of Human Keratinocytes -- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture -- Chap 3 : Isolation and Culture of Epidermal Melanocytes -- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor -- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells -- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts -- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells -- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue -- Part 2 : Tissue engineering skin and engineered skin models -- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility -- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes -- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells -- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes -- Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering -- Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models -- Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin -- Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts -- Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy -- Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo -- Chap 18 : Human Reconstructed Skin in a Mouse Model -- Chap 19 : Pig Model to Test Tissue Engineered Skin -- Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model.
Keywords: Regenerative medicine; Tissue engineering; Cell culture; Regenerative Medicine/Tissue Engineering; Cell Culture